Zeocin生物制品是呈淡綠色的液體，屬于博來(lái)霉素家族的糖蛋白***的一種，Zeocin產(chǎn)品需要儲(chǔ)存在-20℃環(huán)境中。Zeocin主要用于細(xì)胞培養(yǎng)、篩選轉(zhuǎn)化菌株、篩選植物和動(dòng)物細(xì)胞等。
1、產(chǎn)品簡(jiǎn)介
Zeocin可選擇Sh ble基因表達(dá)的細(xì)胞。與現(xiàn)在使用的其他動(dòng)物細(xì)胞標(biāo)記物無(wú)交叉抗性。因此這種***可用來(lái)分離對(duì)其他篩選劑(如：慶大霉素，潮霉素)有抗性的克隆。Zeocin是一種屬于爭(zhēng)光霉素家族的糖蛋白***，在體內(nèi)能作用于大多數(shù)**(包括E。coli)、**(如：酵母菌)、植物細(xì)胞、動(dòng)物細(xì)胞、細(xì)胞培養(yǎng)級(jí)。
2、使用:
●液體Zeocin可用于細(xì)胞培養(yǎng)。
●用于篩選轉(zhuǎn)化株的Zeocin濃度根據(jù)pH值和鹽濃度改變。
●pH越高，鹽濃度越低Zeocin活性越大。
●普通大腸桿菌菌株篩選可在25μg/ml zeocin濃度的低鹽LB瓊脂培養(yǎng)基中進(jìn)行，pH調(diào)至7.5。
●動(dòng)物細(xì)胞篩選：根據(jù)不同細(xì)胞系從100到750μg/ml。
●植物細(xì)胞篩選：根據(jù)不同的細(xì)胞種類和培養(yǎng)基從50到250μg/ml。
●酵母篩選：取決于種屬和培養(yǎng)基，S。cerevisiae Sh ble轉(zhuǎn)化株在50μg/ml濃度的YPD培養(yǎng)基中篩選，pH7.0.304015.
3、Invitrogen相關(guān)資料如下:
Cat. No. R250-01
Zeocin? Selection Reagent
Description
Zeocin? Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin? is prevented from binding and cleaving the cellular DNA. Zeocin? is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin? required for selection will vary between 50-2000 ug/ml (typically 300 ug/ml) depending on the cell type.
C55H83N19O21S2Cu
Formula Weight: 1137.41 g/mole
Zeocin? is a trademark of CAYLA.
The ble resistance gene is the subject of one or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreign equivalents owned by Cayla and licensed to Invitrogen. This product is sold for research purposes only. For commercial license information, please contact: Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.
General Specifications
Concentration: 100 mg?mL
Brand: Zeocin?
Form: Liquid
Product Size: 1 g
Validated Application: Eukaryotic Selection?Stable Cell Line Generation
Bacterial Selection
Contents & Storage
Zeocin? is provided in solution at a concentration of 100 mg/ml. The 1 gram size is supplied in 8 vials. The 5 gram size is supplied in one vial. Store Zeocin? at -20°C in the dark. Zeocin? is guaranteed stable for 6 months when properly stored.
Zeocin的生物制品比較多，很多國(guó)內(nèi)外生物公司都有Zeocin產(chǎn)品，本文主要介紹一下Zeocin的特性、功能及常用于哪些實(shí)驗(yàn)中，并列舉了Invitrogen公司的Zeocin產(chǎn)品，介紹其特點(diǎn)。
