SignaGen生物技術(shù)公司于2006年成立于美國馬里蘭州。公司的創(chuàng)始人系幾個來自美國國立衛(wèi)生研究院(National Institutes of health, NIH)的致力于生物醫(yī)學(xué)研究的科學(xué)家。
自SignaGen生物技術(shù)公司建立的**天起, 他們就一直致力于成為美國基因轉(zhuǎn)導(dǎo)及基因**領(lǐng)域的先驅(qū). 目前SignaGen公司已經(jīng)成功地開發(fā)出三大系列分別用于體內(nèi)和體外的DNA和RNA轉(zhuǎn)染試劑, 是目前世界上**擁有從多聚陽離子聚合物到陽離子型脂質(zhì)體轉(zhuǎn)染試劑的生物公司.
當(dāng)他們還在NIH從事生物醫(yī)學(xué)基礎(chǔ)研究時, 他們就經(jīng)常需要將外源性基因?qū)氲讲溉閯游锛?xì)胞. 他們一方面驚訝于DNA轉(zhuǎn)染試劑出色的轉(zhuǎn)染效率, 另一方面他們也驚訝于轉(zhuǎn)染試劑高的離譜的價格. 從那一刻起, 他們開始嘗試著用老掉牙的旋轉(zhuǎn)蒸發(fā)儀在自己的實驗室制作基于脂質(zhì)體的DNA轉(zhuǎn)染試劑. 經(jīng)過耗時費力的程序及制劑的優(yōu)化工作, 他們自制的DNA轉(zhuǎn)染試劑同市場上好的轉(zhuǎn)染試劑一樣出色, 但是成本只是這些商用轉(zhuǎn)染試劑的零頭. 從那時起, 他們開始質(zhì)疑這些商用轉(zhuǎn)染試劑高得離譜的價格. 他們?yōu)槭裁床豢梢宰约褐谱骱弯N售這些轉(zhuǎn)染試劑? 他們?yōu)槭裁床豢梢詾樗麄兊耐袀兲峁└咝? 低價位的轉(zhuǎn)染試劑呢? 基于這一想法, 他們成立了該公司并開始研制開發(fā)DNA和RNA轉(zhuǎn)染試劑.
2007年1月, 他們向市場投放**個DNA轉(zhuǎn)染試劑------GenJet? 體外DNA轉(zhuǎn)染試劑, 該試劑因為極高的轉(zhuǎn)染效率和極低的毒性迅速獲得生物醫(yī)學(xué)科研工作者的青睞. 接下來他們迅速地升級了該轉(zhuǎn)染試劑, 先后研制出了GenJet?(版本II)和GenJet? Plus等系列**升級產(chǎn)品. 這些升級產(chǎn)品因為顯著地增加了DNA結(jié)合基團, 所以轉(zhuǎn)染效率更高. 近他們又先后研發(fā)成功了基于多聚陽離子聚合物的轉(zhuǎn)染試劑-------PolyJet?體外DNA轉(zhuǎn)染試劑和基于脂質(zhì)體的LipoD293? 體外DNA轉(zhuǎn)染試劑. PolyJet?體外DNA轉(zhuǎn)染試劑一種新型的生物可降解的聚合物, 進入細(xì)胞后, 該聚合物迅速完全地降解, 從而幾乎不產(chǎn)生細(xì)胞毒性; LipoD293?體外DNA轉(zhuǎn)染試劑則為一個加強型DNA轉(zhuǎn)染試劑, 因為其中添加了多肽增強劑, 所以其轉(zhuǎn)染效率非常高, 適用于難以轉(zhuǎn)染的哺乳動物細(xì)胞.
讓我們拭目以待, 看一看接下來的幾個月他們能為您帶來怎樣的驚喜!
SignaGen生物公司, 一個基因轉(zhuǎn)導(dǎo)/基因**公司, 我們的口號: “Combine Efficiency & Affordability..."
美國近出版的生物專業(yè)書刊對SignaGen轉(zhuǎn)染試劑的評價(google)搜索：
1. Run-Qiang Chen, Qing-Kai Yang, Yan-Ling Chen, Vasco A. Oliveira, William S. Dalton, Colleen Fearns, and Jiing-Dwan Lee. Kinome Sirna screen Identifies SMG-1 as a Negative Regulator of Hypoxia-inducible Factor-1α in Hypoxia.. J Biol Chem. 2009; 284(25): 16752–16758.
2. Thomas E. Taylor-Clark, Srinivas Ghatta, Weston Bettner, and Bradley J. Undem. Nitrooleic Acid, an Endogenous Product of Nitrative Stress, Activates Nociceptive Sensory Nerves via the Direct Activation of TRPA1. Molecular Pharmacology, 2009, to be published, available on web
3. Luke C. McSpadden, Robert D. Kirkton, and Nenad Bursac. lectrotonic loading of anisotropic cardiac monolayers by unexcitable cells depends on connexin type and expression level. Am J Physiol Cell Physiol, 2009; 297:C339-C351
4. Run-qiang Chen, Qing-kai Yang, Bing-wen Lu, Wei Yi, Greg Cantin, Yan-ling Chen, Colleen Fearns, John R. Yates, III and Jiing-Dwan Lee. CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells. Cancer Research, 2009; 69:2663
5. Peter Klover, Weiping Chen, Bing-Mei Zhu, and Lothar Hennighausen. Skeletal muscle growth and fiber composition in mice are regulated through the transcription factors STAT5a/b: linking growth hormone to the androgen receptor. FASEB Journal, 2009, to be published, available on web
6. Thomas E. Taylor-Clark, Srinivas Ghatta, Weston Bettner and Bradley J. Undem#. Nitrooleic acid, an Endogenous Product of Nitrative Stress, Activates Nociceptive Sensory Nerves via the Direct Activation of TRPA1. Molecular Pharmacology, 2009, to be published, available on web
7. Krista A. Delviks-Frankenberry, Galina N. Nikolenko, Frank Maldarelli, Saiki Hase, Yutaka Takebe, and Vinay K. Pathak. Subtype-Specific Differences in the Human Immunodeficiency Virus Type 1 Reverse Transcriptase Connection Subdomain of CRF01_AE Are Associated with Higher Levels of Resistance to 3'-Azido-3'-Deoxythymidine. Journal of Virology, September 2009, 83: 8502-8513
8. Bin Jia, Ruth Serra-Moreno, William Neidermyer, Jr., Andrew Rahmberg, John Mackey, Ismael Ben Fofana, Welkin E. Johnson, Susan Westmoreland, David T. Evans. Species-Specific Activity of SIV Nef and HIV-1 Vpu in Overcoming Restriction by Tetherin/BST2. PLoS Pathogens, 2009, to be published, available on web
9. Wen-Bin Tsai, Young Min Chung, Yoko Takahashi, Zhaohui Xu, and Mickey C-T. Hu. Functional interaction between FOXO3a and ATM regulates DNA damage response. Nat Cell Biol. 2008; 10(4): 460–467
10. Rothenburg S, Seo EJ, Gibbs JS, Dever TE, Dittmar K.. Rapid evolution of protein kinase PKR alters sensitivity to viral inhibitors. Nat Struct Mol Biol. 2009; 16(1):63-70
11. J-H Zhu, R Chen, W Yi, G T Cantin, C Fearns, Y Yang, J R Yates III and J-D Lee. Protein tyrosine phosphatase PTPN13 negatively regulates Her2/ErbB2 malignant signaling. Oncogene 2008; 27: 2525-31
12. Krista A. Delviks-Frankenberry, Galina N. Nikolenko, Paul L. Boyer, Stephen H. Hughes, John M. Coffin, Abhay Jere, and Vinay K. Pathak. HIV-1 reverse transcriptase connection subdomain mutations reduce template RNA degradation and enhance AZT excision. Proc Natl Acad Sci U S A. 2008; 105: 10943–10948
13. Jianhua Zhao, Zhenping Zhang, Zivjena Vucetic, Kenneth J. Soprano, Dianne Robert Soprano. HACE1: A novel repressor of RAR transcriptional activity. Journal of Cellular Biochemistry, 2008; 107: 482-493
14. Rebecca Klinger and Nenad Bursac. Cardiac Cell Therapy In Vitro: Reproducible Assays for Comparing the Efficacy of Different Donor Cells. IEEE Eng Med Biol Mag. Author manuscript; available in PMC 2009 July 24
15. Shiling Yang, Shouzou Wei, Ambra Pozzi and Jorge H. Capdevila. The arachidonic acid epoxygenase is a component of the signaling mechanisms responsible for VEGF-stimulated angiogenesis. Archives of Biochemistry and Biophysics, 2009 to be published, available on web
16. SALLY DABELSTEEN,a,b PAULA HERCULE,a PATRICIA BARRON,a MEGHAN RICE, GREGORY DORSAINVILLE, JAMES G. RHEINWALD. Epithelial Cells Derived from Human Embryonic Stem Cells Display P16INK4A Senescence, Hypermotility, and Differentiation Properties Shared by Many P631 Somatic Cell Types. STEM CELLS, 2009;27:1388–1399
17. Boris B. Boyanovsky1, Preetha Shridas2, Michael Simons, Deneys R. van der Westhuyzen1,2,3, and Nancy R. Webb. Syndecan-4 Mediates Macrophage Uptake of Group V Secretory Phospholipase A2-Modified Low Density Lipoprotein. J Lipid Research, 2009 to be published, available on web
18. Young Yil Bahk, Ick-Hyun Cho and Tong Soo Kim. A Cross-talk between oncogenic Ras and tumor suppressor PTEN through FAK Tyr861 phosphorylation in NIH/3T3 mouse embryonic fibroblasts. Biochemical and Biophysical Research Communications, 2008; 377: 1199-1204
19. Grzegorz Woszczek, Li-Yuan Chen, Sahrudaya Nagineni, Sara Alsaaty, Anya Harry, Carolea Logun, Rafal Pawliczak, and James H. Shelhamer. IFN- Induces Cysteinyl Leukotriene Receptor 2 Expression and Enhances the Responsiveness of Human Endothelial Cells to Cysteinyl Leukotrienes. The Journal of Immunology, 2007;178: 5262-5270
20. Jean-Marie Sontag,1 Viyada Nunbhakdi-Craig,1 Lisa Montgomery,1 Erland Arning,2 Teodoro Bottiglieri,2 and Estelle Sontag. Folate Deficiency Induces In Vitro and Mouse Brain Region-Specific Downregulation of Leucine Carboxyl Methyltransferase-1 and Protein Phosphatase 2A B_ Subunit Expression That Correlate with Enhanced Tau Phosphorylation. The Journal of Neuroscience, 2008; 28(45):11477–11487
21. Jacob, Abraham; Lee, Tina X.; Neff, Brian A.; Miller, Shyra; Welling, Bradley; Chang, Long-Sheng. Phosphatidylinositol 3-Kinase/AKT Pathway Activation in Human Vestibular Schwannoma. Otology & Neurotology, 2008; 29: 58-68
22. Rachel A. Karlnoski, Arnon Rosenthal, Dione Kobayashi, Jaume Pons, Jennifer Alamed, Mary Mercer, Qingyou Li, Marcia N. Gordon, Paul E. Gottschall, and David Morgan. Suppression of Amyloid Deposition Leads to Long-Term Reductions in Alzheimer's Pathologies in Tg2576 Mice. The Journal of Neuroscience, 2009, 29(15):4964-4971
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美國SignaGen公司官方網(wǎng)站：http://signagen.com/
大陸地區(qū)總代理： http://www.jnqsw.com/
